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KMID : 0380619870190050441
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Korean Journal of Food Science and Technology
1987 Volume.19 No. 5 p.441 ~ p.445
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Localization of Lipoxygenase in Germinating Soybeans
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Abstract
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The subcellular distribution of lipoxygenase in germinating soybean seeds (Glycine max [L.] AmSoy) was investigated by using differential centrifugation and sucrose density gradient fractionation. Most of lipoxygenase -1 and -2/3 activities was present in the supernatant fraction after differential centrifugation of homogenates prepared from three-day-old seedlings; only 1.5% of lipoxygenase activity remained in particulate fractions. The results of a sucrose density gradient fractionation (three-day-old) showed that the lipoxygenase activity coincided with acid phosphatase at the densities of 1.19, 1.23, 1.25 g/㎤, even though most of lipoxygenase and acid phosphatase activities appeared in supernatant fractions. There was no indication that mitochondria contained any lipoxygenase activity, and it does not appear that glyoxysomes and ER contained any lipoxygenase activity either.
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